CIP29 Antibody - BSA Free

Western Blot: CIP29 Antibody [NBP2-97653] - Anti-CIP29 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 34 kDa

Immunohistochemistry-Paraffin: CIP29 Antibody [NBP2-97653] - Immunochemical staining of human CIP29 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: CIP29 Antibody [NBP2-97653] - Immunochemical staining of human CIP29 in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: CIP29 Antibody [NBP2-97653] - CIP29 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-CIP29 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CIP29 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 34 kDa

Immunocytochemistry/Immunofluorescence: CIP29 Antibody [NBP2-97653] - Immunofluorescence staining of CIP29 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97653 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CIP29 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human CIP29 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SARNP
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CIP29 Antibody - BSA Free

• CIP29Hcc-1
• cytokine induced protein 29 kDa
• Cytokine-induced protein of 29 kDa
• HCC1MGC14726
• hepatocellular carcinoma 1
• HSPC316
• Nuclear protein Hcc-1
• proliferation associated cytokine-inducible protein CIP29
• Proliferation-associated cytokine-inducible protein CIP29
• SAP domain containing ribonucleoprotein
• SAP domain-containing ribonucleoprotein
• THO1

Background

The CIP29 gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a rolein cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting inexpression of a chimeric

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SARNP