CHMP4C Overexpression Lysate (Native)

Images

 
Western Blot: CHMP4C Overexpression Lysate (Adult Normal) [NBL1-09161] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHMP4C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CHMP4C Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204802 and based on accession number NM_152284. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromatin modifying protein 4C (CHMP4C), mRNA.
Gene
CHMP4C

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CHMP4C

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CHMP4C Overexpression Lysate (Native)

  • charged multivesicular body protein 4c
  • CHMP4c
  • chromatin modifying protein 4C
  • Chromatin-modifying protein 4c
  • hSnf7-3
  • hVps32-3
  • MGC22825
  • Shax3
  • SNF7 homolog associated with Alix 3
  • Snf7 homologue associated with Alix 3
  • SNF7-3
  • Vacuolar protein sorting-associated protein 32-3
  • vps32-3
  • VPS32C

Background

CHMP4C belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CHMP4C