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Recombinant Human CHMP4A His Protein

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SDS-Page: Recombinant Human CHMP4A His Protein [NBP1-78875] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human CHMP4A His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-265 of Human CHMP4A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CHMP4A
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 50% glycerol, 200 mM NaCl, 0.1 mM PMSF, 1 mM EDTA
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human CHMP4A His Protein

  • C14orf123
  • charged multivesicular body protein 4a
  • CHMP4
  • CHMP4a
  • CHMP4B
  • chromatin modifying protein 4A
  • Chromatin-modifying protein 4a
  • chromosome 14 open reading frame 123
  • FLJ61658
  • hSnf-1
  • HSPC134
  • hVps32-1
  • MGC142093
  • MGC142095
  • SHAX2
  • SNF7 homolog associated with Alix-2
  • Snf7 homologue associated with Alix 2
  • SNF7
  • SNF7-1
  • Vacuolar protein sorting-associated protein 32-1
  • VPS32-1
  • VPS32A

Background

CHMP4A (Chromatin-modifying protein 4a) belongs to the SNF7 family and functions as chromatin-modifying proteins. It probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, which is present in viral Gag assembly and budding. CHMP4A expressed at higher level in heart, kidney, liver and skeletal muscle. Recombinant human CHMP4A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CHMP4A