Novus Biologicals products are now on bio-techne.com

CHMP4A Recombinant Protein Antigen

Images

 
There are currently no images for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

CHMP4A Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CHMP4A.

Source: E. coli

Amino Acid Sequence: THLPAGPAPKVDEDEEALKQLAEWVS

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
CHMP4A
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49557.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking tide related information and a protocol, click here.

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CHMP4A Recombinant Protein Antigen

  • C14orf123
  • charged multivesicular body protein 4a
  • CHMP4
  • CHMP4a
  • CHMP4B
  • chromatin modifying protein 4A
  • Chromatin-modifying protein 4a
  • chromosome 14 open reading frame 123
  • FLJ61658
  • hSnf-1
  • HSPC134
  • hVps32-1
  • MGC142093
  • MGC142095
  • SHAX2
  • SNF7 homolog associated with Alix-2
  • Snf7 homologue associated with Alix 2
  • SNF7
  • SNF7-1
  • Vacuolar protein sorting-associated protein 32-1
  • VPS32-1
  • VPS32A

Background

CHMP4A (charged MVB protein 4A) is one of the three isoforms of CHMP4, the human equivalent of yeast Snf7 (so CHMP4A is also referred to as hSnf7-1). It is a component of the ESCRT-III (endosomal sorting complex required for transport III) complex. The ESCRT complexes mediate membrane budding away from the cytosol and cleavage of membrane necks (membrane fission) from the cytosolic face; hence, they are key in processes such as the formation of MVBs (multivesicular bodies), enveloped virus budding, and membrane abscission in cytokinesis. Not all types of ESCRT complexes, or proteins within those complexes, are necessary for all of these functions. For instance, research into the requirements of ESCRT-III proteins in HIV-1 budding has revealed that CHMP1 and CHMP3 proteins are not essential for this process; at least one member of each of the CHMP2 and CHMP4 families are required; within these families there is some functional redundancy; within the CHMP4 family, the relative importance and abundance of the isoforms is 4B > 4A > 4C; and the absence of CHMP4A or 4B prevents the budding process from progressing to completion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90201
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP3-27784
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP1-83726
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-91780
Species: Hu
Applications: IHC, IHC-P
NB200-112
Species: Ca, Ha, Hu, Pm, Mu, Po, Pm, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, IP, KD, WB
NBP2-20881
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-22183
Species: Hu, Mu, Rt
Applications: Flow, IHC, IP, WB
AF6015
Species: Hu, Mu, Rt
Applications: WB
NBP1-81166
Species: Hu
Applications: IHC, IHC-P
NBP1-86745
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF7117
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP2-32665
Species: Hu
Applications: IHC, IHC-P, WB
AF8171
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-15278
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-77198
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
NBP1-30081
Species: Hu, Mu
Applications: IP, WB
H00008086-M02
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-90706
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB

Publications for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP) (0)

There are no publications for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP) (0)

There are no reviews for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CHMP4A Recombinant Protein Antigen (NBP2-49557PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CHMP4A Products

Blogs on CHMP4A

There are no specific blogs for CHMP4A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our CHMP4A Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol CHMP4A