Chitotriosidase/CHIT1 Antibody (1D9G2)

Western Blot: Chitotriosidase/CHIT1 Antibody (1D9G2) [NBP1-28895] - Western blot analysis using CHIT1 mouse mAb against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).

Product Discontinued

Chitotriosidase/CHIT1 Antibody (1D9G2) Summary

• Immunogen: Purified recombinant fragment of CHIT1(aa22-137) expressed in E. Coli.
• Specificity: Specific for CHIT1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHIT1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Western Blot 1:500-1:2000
• Application Notes: Use in Flow-intracellular and Flow-cell surface reported in scientific literature (PMID: 22532562).

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Chitotriosidase/CHIT1 Antibody (1D9G2)

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase is a 50 kDa secreted glycoprotein that belongs to glycohydrolase family # 18. It is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chitotriosidase/CHIT1 Antibody (NBP1-28895)(1)

Publication using NBP1-28895

• Berger M, Hsieh CY, Bakele M et al. Neutrophils express distinct RNA receptors in a non-canonical way. J Biol Chem. 2012-06-01 [PMID: 22532562] (Flow-CS, Flow Cytometry Control)
  
  Details:
  TLR8-PE (IMG-321D): Flow (intracellular) + Flow (cell surface), Fig 1A (human neutrophils) & Fig 1B (HL60).2. TLR8 (IMG-321A): IF confocal microscopy, Fig 1C (human neutrophils).3. FITC-conjugated goat anti-rabbit secondary Ab (Cat no 20302), Flow (intracellular) + Flow (cell surface), various Figs.4. FITC-conjugated donkey anti-rat secondary Ab (Cat no 20204), Flow (intracellular) + Flow (cell surface), various Figs.

Bioinformatics

• Gene Symbol: CHIT1