Chitinase 3-like 2 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Chitinase 3-like 2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 2
  Tyr27-Leu390
  Accession # Q15782
• Specificity: Detects human Chitinase 3-like 2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human CHIT-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CHI3L2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitinase 3-like 2 Antibody [Biotin]

• CHI3L2
• Chitinase 3 like 2
• Chitinase 3-like 2
• chitinase-3-like protein 2
• Chondrocyte protein 39
• YKL39
• YKL-39YKL39

Background

Chitinase 3-like 2 (CHI3L2), also called chondrocyte protein 39 or YKL-39, is a 39 kDa potentially glycosylated member of the glycosyl hydrolase 18 family (1‑3). It is secreted by synovial fibroblasts, chondrocytes, and TGF beta /IL-4-stimulated monocyte-derived macrophages (1, 4, 5). The human CHI3L2 cDNA encodes 390 amino acids (aa), including a 26 aa signal sequence and a 364 aa mature region. Potential alternate start sites at Met-24 and Met+80 would create isoforms of 413 aa and 311 aa, while a potential deletion of aa 15‑24 would create a 380 aa isoform (6). Mature human CHI3L2 shares 89%, 87% and 84% aa sequence identity with bovine, porcine and equine CHI3L2, respectively. The rodent genome does not include a CHI3L2 ortholog (2). Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑3). Unlike CHI3L1, CHI3L2 does not bind heparin (1). While CHI3L1 is the major protein excreted by chondrocytes, up-regulation of CHI3L2 and not CHI3L1 correlates with osteoarthritic cartilage degeneration (1, 7, 8). CHI3L2 has been proposed as a potential biomarker for osteoarthritis (4, 7, 8). CHI3L1 has been found to coordinate adhesion receptors and promote cell signaling and tumor angiogenesis; like CHI3L1, CHI3L2 can enhance cell adhesion (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHI3L2
• Uniprot:
  • Q15782(Human)