CHD4 Antibody (3F2/4) - Azide and BSA Free

Western Blot: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).

Immunocytochemistry/Immunofluorescence: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.

Immunohistochemistry-Paraffin: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3F2/4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CHD4 Antibody (3F2/4) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07505).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunization: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognizes has not been tested. (Uniprot: Q14839)
• Localization: Nucleus and cytoplasm.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHD4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
• Theoretical MW: 238 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CHD4 Antibody (3F2/4) - Azide and BSA Free

• ATP-dependent helicase CHD4
• CHD-4
• chromodomain helicase DNA binding protein 4
• chromodomain-helicase-DNA-binding protein 4
• DKFZp686E06161
• EC 3.6.1
• EC 3.6.4.12
• Mi-2 autoantigen 218 kDa protein
• Mi-2b
• Mi2-BETA

Background

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHD4