CHD1L Antibody (JG35-44)

Western Blot: CHD1L Antibody (JG35-44) [NBP2-76984] - Western blot analysis of CHD1L on HepG2 cell using anti-CHD1L antibody at 1/1,000 dilution.

Immunohistochemistry: CHD1L Antibody (JG35-44) [NBP2-76984] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval

Flow Cytometry: CHD1L Antibody (JG35-44) [NBP2-76984] - Flow cytometric analysis of HepG2 cells with CHD1L antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: CHD1L Antibody (JG35-44) [NBP2-76984] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry: CHD1L Antibody (JG35-44) [NBP2-76984] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Product Discontinued

CHD1L Antibody (JG35-44) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CHD1L aa 680-820 / 897. (SwissProt: Q86WJ1 Human; SwissProt: Q9CXF7 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHD1L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CHD1L Antibody (JG35-44)

• ALC1
• amplified in liver cancer 1
• amplified in liver cancer protein 1
• CHD1L
• CHDL
• chromodomain helicase DNA binding protein 1-like
• chromodomain-helicase-DNA-binding protein 1-like
• FLJ22530

Background

In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 (PubMed 19661379)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHD1L