cGK1/PRKG1 Antibody

Images

 
Western Blot: cGK1/PRKG1 Antibody [NBP2-14843] - Lane 1: MW marker, Lane 2: HeLa cell lysate, Lane 3: Rat brain tissue extract, Lane 4: MDBK cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

cGK1/PRKG1 Antibody Summary

Immunogen
Synthetic peptide corresponding to a portion of human PKG1B, with the subsitiution of a tryptophan residue for tyrosine at residue 9.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-14843 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for cGK1/PRKG1 Antibody

  • AAT8
  • cGK 1
  • cGK
  • cGK1
  • cGK1/PRKG1
  • cGKI
  • cGKI-alpha
  • cGKI-BETA
  • cGMP-dependent protein kinase I
  • DKFZp686K042
  • EC 2.7.11
  • EC 2.7.11.12
  • FLJ36117
  • PKG
  • PKG1
  • PRKG1
  • PRKG1B
  • PRKG1BMGC71944
  • PRKGR1A
  • PRKGR1B
  • PRKGR1BcGMP-dependent, regulatory, type I, beta
  • protein kinase cGMP-dependent 1
  • protein kinase, cGMP-dependent, type I

Background

cGK1/PRKG1, also known as cGMP-dependent protein kinase 1, has two isoforms that are produced by alternative splicing. Isoform Alpha (CGK1-alpha) is 671 amino acids long and 76 kDa. Isoform Beta (CGK1-beta) is 686 amino acids long and approximately 78 kDa. PRKG1 is a Serine/threonine protein kinase that is expressed in placenta and lung. cGK1/PRKG1 is an important player in the Nitric Oxide/cGMP Signaling Pathway; the binding of GMP activates PRKG1, which in turn phosphorylates Serine residues and Threonine residues on various cell proteins, including proteins that regulate calcium levels, smooth muscle contraction, platelet adhesion, circadian rhythm and more. Current research on PRKG1 is being conducted in relation to several diseases and disorders including cystic fibrosis, lymphoma, non-small cell lung carcinoma, Wiskott-Aldrich syndrome, Waterhouse-Friderichsen syndrome and myoglobinuria. PRKG1 has also been shown to have interactions with SMAD4, BMPR2, GKAP1, Natriuretic Peptide Receptor A and TFII-I in pathways such as Gap Junction signaling, eNOS signaling and EDNRB signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for cGK1/PRKG1 Antibody (NBP2-14843) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-14843:
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Images Ratings Applications Species Date Details
Western Blot cGK1/PRKG1 NBP2-14843
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5
reviewed by:
Verified Customer
WB Mouse 10/11/2019
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Summary

ApplicationWestern Blot
Sample TestedEndothelial
SpeciesMouse
Lot09061834

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Verified Customer
10/11/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PRKG1