CENPQ Antibody

Images

 
Western Blot: CENPQ Antibody [NBP1-42740] - Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus.
Immunocytochemistry/ Immunofluorescence: CENPQ Antibody [NBP1-42740] - Analysis of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red ...read more

Product Details

Summary
Reactivity Hu, Other, PmSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CENPQ Antibody Summary

Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Specificity
This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPQ
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • ELISA 1:5000 1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This protein A purified antibody has been tested for use in ELISA, Immunofluorescence microscopy and western blotting.Expect a band approximately 26-31 kDa in size corresponding - human CENP-Q by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

hu, macaque, other

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for CENPQ Antibody

  • C6orf139
  • CENP-QFLJ10545
  • centromere protein Q
  • chromosome 6 open reading frame 139

Background

Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CENPQ
Entrez