CENPM Antibody (4C12-2C8)

Images

 
Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Analysis of C22orf18 expression in K-562.
Immunocytochemistry/ Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: ...read more
Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit ...read more
ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01] - Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
4C12-2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CENPM Antibody (4C12-2C8) Summary

Immunogen
C22orf18 (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Localization
Cytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres.
Specificity
C22orf18 - chromosome 22 open reading frame 18
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CENPM
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CENPM Antibody (4C12-2C8)

  • bK250D10.2
  • C22orf18PANE1MGC861
  • CENP-Mchromosome 22 open reading frame 18
  • centromere protein M
  • ICEN39
  • Interphase centromere complex protein 39
  • Pane1
  • proliferation associated nuclear element 1
  • Proliferation-associated nuclear element protein 1

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CENPM
Entrez
Uniprot