CENPM Antibody (4C12-2C8) - Azide and BSA Free

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Analysis of C22orf18 expression in K-562.

Immunocytochemistry/Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.

ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01] - Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 4C12-2C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CENPM Antibody (4C12-2C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CENPM Antibody (4C12-2C8) - Azide and BSA Free (H00079019-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C22orf18 (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
• Localization: Cytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres.
• Specificity: C22orf18 - chromosome 22 open reading frame 18
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CENPM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CENPM Antibody (4C12-2C8) - Azide and BSA Free

• bK250D10.2
• C22orf18PANE1MGC861
• CENP-Mchromosome 22 open reading frame 18
• centromere protein M
• ICEN39
• Interphase centromere complex protein 39
• Pane1
• proliferation associated nuclear element 1
• Proliferation-associated nuclear element protein 1

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CENPM
• Entrez:
  • 79019(Human)
• Uniprot:
  • AAH00705(Human)