Mouse CENPH ELISA Kit (Colorimetric)

ELISA: Mouse CENPH ELISA Kit (Colorimetric) [NBP2-75233] - Samples were spiked with high concentrations of Mouse CENPH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse CENPH ELISA Kit (Colorimetric) [NBP2-75233] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse CENPH ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CENPH in samples. No significant cross-reactivity or interference between Mouse CENPH and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.83%
• Intra-Assay: CV% < 4.83%
• Spike Recovery: 89-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CENPH

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CENPH ELISA Kit (Colorimetric)

• CENP-H
• centromere protein H
• ICEN35
• Interphase centromere complex protein 35
• kinetochore protein CENP-H
• NNF1
• PMF1

Background

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CENPH