CEACAM1/CD66a Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) ...read more
Mouse splenocytes were stained with Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ...read more
CEACAM‑1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CEACAM1/CD66a Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a
Met1-Gly428
Accession # P31809
Specificity
Detects mouse CEACAM-1/CD66a in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Ceacam1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF6480 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM1/CD66a Antibody [Unconjugated]

  • antigen CD66
  • BGP1
  • BGP-1
  • BGP1BGPBiliary glycoprotein 1
  • BGPI
  • Biliary Glycoprotein 1
  • biliary glycoprotein adhesion molecule
  • carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
  • carcinoembryonic antigen-related cell adhesion molecule 1
  • CD66a antigen
  • CD66a
  • Cea-1
  • CEACAM1
  • CEACAM-1
  • Hv-1
  • Hv-2
  • MHVR

Background

CEACAM-1 (Carcinoembryonic antigen-related cell adhesion molecule 1; also BGP-1, CD66a and MHVR1) is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type
(aa 147-411) Ig-like domains, plus a 74 aa cytoplasmic domain. Three alternate splice forms exist. One contains a four aa substitution for aa 455-521, a second shows a Gln substitution for aa 142-322, and a third possesses a combination of the first two splice patterns. CEACAM-1 forms homodimers. Over aa 35-428, mouse CEACAM-1 shares 56% and 70% aa identity with human and rat CEACAM-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEACAM1/CD66a Antibody (AF6480)(3)

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Bioinformatics

Gene Symbol Ceacam1
Uniprot