Human CDK8 ELISA Kit (Colorimetric)

ELISA: Human CDK8 ELISA Kit (Colorimetric) [NBP2-75179] - Samples were spiked with high concentrations of Human CDK8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human CDK8 ELISA Kit (Colorimetric) [NBP2-75179] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human CDK8 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CDK8 in samples. No significant cross-reactivity or interference between Human CDK8 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.63%
• Intra-Assay: CV% < 5.27%
• Spike Recovery: 86-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CDK8

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CDK8 ELISA Kit (Colorimetric)

• CDK8 protein kinase
• CDK8
• Cell division protein kinase 8
• cyclin-dependent kinase 8
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• K35
• Mediator complex subunit CDK8
• Mediator of RNA polymerase II transcription subunit CDK8
• MGC126074
• MGC126075
• Protein kinase K35

Background

Cdk8 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CDK8 ELISA Kit (NBP2-75179). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: CDK8