Cdk6 Antibody

Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP2-15846] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK6 protein stained by CDK6 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Cdk6 Antibody [NBP2-15846] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Cdk6 Antibody [NBP2-15846] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] diluted at 1:500.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cdk6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cdk6. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%), Rhesus Macaque. Bovine reactivity reported in scientific liteRature (PMID:31987327).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cdk6 Antibody

• Cdk6
• CDKN6
• Cell division protein kinase 6
• cyclin-dependent kinase 6
• EC 2.7.11
• EC 2.7.11.22
• PLSTIREMGC59692
• Serine/threonine-protein kinase PLSTIRE

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cdk6 Antibody (NBP2-15846)(2)

Publications using NBP2-15846

• Liu B, Fu C, Cao J Et al. Proliferation of bovine endometrial epithelial cells is promoted by prostaglandin E2-PTGER2 signaling through cell cycle regulation Prostaglandins, Leukotrienes and Essential Fatty Acids 2021-11-01 [PMID: 34740034] (WB, Bovine)
• Fu C, Mao W, Gao R et al. Prostaglandin F2 alpha-PTGFR signaling promotes proliferation of endometrial epithelial cells of cattle through cell cycle regulation Anim. Reprod. Sci. 2020-02-01 [PMID: 31987327] (WB, Bovine)

Bioinformatics

• Gene Symbol: CDK6
• Uniprot:
  • Q00534(Human)