CDK4 Antibody (4F11) - Azide and BSA Free

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.

ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 4F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CDK4 Antibody (4F11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDK4 (AAH03644, 211 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE
• Specificity: CDK4 (4F11)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDK4 Antibody (4F11) - Azide and BSA Free

• CDK4
• Cell division protein kinase 4
• CMM3
• cyclin-dependent kinase 4
• EC 2.7.11
• EC 2.7.11.22
• melanoma cutaneous malignant, 3
• MGC14458
• PSK-J3
• PSK-J3cell division kinase 4

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK4
• Entrez:
  • 1019(Human)
• OMIM:
  • 123829(Human)
• Uniprot:
  • AAH03644(Human)