CDC37 Antibody

Western Blot: CDC37 Antibody [NBP1-70346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CDC37 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC37 was also immunoprecipitated by another rabbit anti-CDC37 antibody , which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CDC37 Antibody Summary

• Description: Novus Biologicals Rabbit CDC37 Antibody (NBP1-70346) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 324 to 374 of human cell division cycle 37 using the numbering given in entry NP_008996.1 (GeneID 11140).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC37
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC37 Antibody

• CDC37 (cell division cycle 37, S. cerevisiae, homolog)
• CDC37 cell division cycle 37 homolog (S. cerevisiae)
• CDC37 cell division cycle 37 homolog
• CDC37A
• cell division cycle 37 homolog (S. cerevisiae)
• Hsp90 chaperone protein kinase-targeting subunit
• hsp90 co-chaperone Cdc37
• P50CDC37

Background

Cell division cycle 37 protein (CDC37) is also known as Hsp90 co-chaperone Cdc37. CDC37 is highly similar to Cdc37, a cell division cycle control protein of Saccharomyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form a complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [taken from NCBI Entrez Gene (GeneID: 11140)].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC37