CDC2/CDK1 [p Thr161] Antibody

Product Discontinued

CDC2/CDK1 [p Thr161] Antibody Summary

• Immunogen: synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T-Y-TP-H-E).
• Modification: p Thr161
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC2/CDK1 [p Thr161] Antibody

• CDC2
• CDC28A
• CDC2MGC111195
• CDK1
• cell cycle controller CDC2
• Cell division control protein 2 homolog
• Cell division protein kinase 1
• cyclin-dependent kinase 1
• DKFZp686L20222
• EC 2.7.11.22
• EC 2.7.11.23
• G1 to S and G2 to M
• p34 protein kinase
• P34CDC2

Background

p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphor-ylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC2/CDK1 Antibody (NBP1-41770)(1)

Publication using NBP1-41770

• Werle K, Chen J, Xu HG et al. Liver kinase B1 regulates the centrosome via PLK1. Cell Death Dis 2014-04-11 [PMID: 24722282] (WB, Human)

Bioinformatics

• Gene Symbol: CDK1