CDC16 Antibody

Immunohistochemistry-Paraffin: CDC16 Antibody [NBP1-02803] - Staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

CDC16 Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region surrounding pT580 of human CDC16.
• Specificity: This antibody is specific for phosphorylated human APC6 protein at the pT580 residue. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:500-1:3000
• Application Notes: IHC: NBP1-02803 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 0.97 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC16 Antibody

• ANAPC6subunit 6
• CDC16 (cell division cycle 16, S. cerevisiae, homolog)
• CDC16 cell division cycle 16 homolog (S. cerevisiae)
• CDC16 homolog
• CDC16Hs
• cell division cycle 16 homolog (S. cerevisiae)
• cell division cycle protein 16 homolog
• Cyclosome subunit 6

Background

APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is composed of at least 11 subunits. APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. Splice variants have been described for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC16
• Entrez:
  • 8881(Human)
• Uniprot:
  • Q13042(Human)