CD96 Antibody

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5690) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 170 - 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Product Discontinued

CD96 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD96
  Glu25-Met536
  Accession # AAH52865
• Specificity: Detects mouse CD96 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD96 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CD96
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD96 Antibody

• CD96 antigenMGC22596
• CD96 molecule
• CD96
• DKFZp667E2122
• T cell-activated increased late expression protein
• TACTILE
• TACTILEincreased late expression
• T-cell surface protein tactile

Background

Mouse CD96 (also Tactile) is a 170‑180 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma δ T cells. Mouse CD96 binds to CD155 and nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22‑536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V‑type (aa 24‑244), while the distal domain is a C-type structure (aa 250‑355). Unlike human, there is no splice variant in the second V‑type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437‑602. Over aa 1‑536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD96
• Uniprot:
  • AAH52865()