CD79B Antibody (683023)

Human peripheral blood lymphocytes were stained with Human CD79B Monoclonal Antibody (Catalog # MAB6620) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on normal mouse IgM control antibody staining .

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 683023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD79B Antibody (683023) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD79B
  Ala29-Asp159
  Accession # P40259
• Specificity: Detects human CD79B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD79B or recombinant human CD79A is observed.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD79B
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD79B Antibody (683023)

• B29
• B29B-cell-specific glycoprotein B29
• B-cell antigen receptor complex-associated protein beta chain
• CD79B antigen (immunoglobulin-associated beta)
• CD79b antigen
• CD79b molecule, immunoglobulin-associated beta
• CD79B
• IGB
• IGBAGM6
• Ig-beta
• Immunoglobulin-associated B29 protein

Background

CD79B, also known as Igb and B29, is a 36 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig‑like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform that lacks nearly the entire Ig‑like domain. Heterodimers of CD79A/Iga and CD79B associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD (aa 29-159), human CD79B shares 54% aa sequence identity with mouse CD79B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD79B
• Uniprot:
  • P40259()