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Human CD74 ELISA Kit (Colorimetric)

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Human CD74 ELISA Kit (Colorimetric) - Standard Curve Reference
Human CD74 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

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Human CD74 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CD74 in samples. No significant cross-reactivity or interference between Human CD74 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.93%
Intra-Assay
CV% < 4.84%
Spike Recovery
87-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD74

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Stop Solution
  2. Substrate Reagent
  3. Micro ELISA Plate(Dismountable)
  4. HRP Conjugate Diluent
  5. Reference Standard & Sample Diluent
  6. Reference Standard
  7. Plate Sealer
  8. Product Description
  9. Concentrated Biotinylated Detection Ab (100×)
  10. Certificate of Analysis
  11. Concentrated HRP Conjugate (100×)
  12. Concentrated Wash Buffer (25×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Human CD74 ELISA Kit (Colorimetric)

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAG
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLADG
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
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Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.
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(OTI2D9)
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Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.
Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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NBP2-67309
Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: HCT116 Lane 3: CRC
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
Species: Hu, Rt
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Advillin Antibody
NBP2-34118
Immunohistochemistry-Paraffin: Advillin Antibody [NBP2-34118] - Staining of human duodenum shows strong cytoplasmic positivity in neuroendocrine cells.
Immunohistochemistry-Paraffin: Advillin Antibody [NBP2-34118] - Staining of human lymphoid tissues shows no positivity as expected.
Immunohistochemistry-Paraffin: Advillin Antibody [NBP2-34118] - Staining of human stomach shows strong cytoplasmic positivity in neuroendocrine cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P

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CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.
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  Human CD74 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CD74