CD74 Antibody (3D7)

Images

 
Western Blot: CD74 Antibody (3D7) [NBP2-59697] - Analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Lane 1: Molecular ...read more
Flow Cytometry: CD74 Antibody (3D7) [NBP2-59697] - Analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
3D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CD74 Antibody (3D7) Summary

Immunogen
Human CD74 invariant chain synthetic peptide.
Localization
Cell Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Golgi Apparatus, Endosome, Lysosome
Specificity
Detects approx 80, 55kDa. No cross-reactivity against Cav Beta4.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD74
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:50
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of CD74 in 15 ug of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4, 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD74 Antibody (3D7)

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAG
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLADG
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33

Background

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment.Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-59697AF405
Alexa Fluor 488 NBP2-59697AF488
Alexa Fluor 647 NBP2-59697AF647
Alexa Fluor 700 NBP2-59697AF700
Allophycocyanin NBP2-59697APC
Allophycocyanin/Cy7 NBP2-59697APCCY7
Biotin NBP2-59697B
DyLight 350 NBP2-59697UV
DyLight 405 NBP2-59697V
DyLight 488 NBP2-59697G
DyLight 550 NBP2-59697R
DyLight 650 NBP2-59697C
DyLight 680 NBP2-59697FR
DyLight 755 NBP2-59697IR
FITC NBP2-59697F
HRP NBP2-59697H
Janelia Fluor 549 NBP2-59697JF549
Janelia Fluor 646 NBP2-59697JF646
PE NBP2-59697PE
PE/Atto594 NBP2-59697PEATT594
PE/Cy5.5 NBP2-59697PECY55
PE/Cy7 NBP2-59697PECY7
PerCP NBP2-59697PCP

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Blogs on CD74.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

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Bioinformatics

Gene Symbol CD74