CD72.1 Antibody (10-1.D.2) [FITC]

Images

 
Flow Cytometry: CD72.1 Antibody (10-1.D.2) [FITC] [NBP1-28023] - BALB/c spleen cells were double-stained with mouse anti-mouse CD72.1-FITC and rat anti-mouse CD19-RPE. Lymphocytes were then gated and analyzed by flow ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, In vitro
Clone
10-1.D.2
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

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Catalog# & Conjugate Size Price

CD72.1 Antibody (10-1.D.2) [FITC] Summary

Immunogen
DBA/2 mouse spleen cells
Specificity
Mouse CD72.1/Lyb-2.1, 45 kDa
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
Cd72
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • In vitro assay

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Conjugated forms should be protected from prolonged exposure to light.

Alternate Names for CD72.1 Antibody (10-1.D.2) [FITC]

  • CD72 antigen
  • Ly19
  • Ly32
  • Lyb2
  • Lym19

Background

Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. (1,2) The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. (1) However, 10-1.D.2 can itself trigger a positive signal by binding CD72.1-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28024

Additional CD72.1 Products

CD72.1 NBP1-28023

Bioinformatics Tool for CD72.1 Antibody (NBP1-28023)

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Bioinformatics

Gene Symbol Cd72