CD39L4/ENTPD5 Antibody (743512)

Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD39L4/ENTPD5 Monoclonal Antibody (Catalog # MAB5297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 743512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39L4/ENTPD5 Antibody (743512) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5
  Val21-His428
  Accession # O75356
• Specificity: Detects human CD39L4/ENTPD5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD39, rhCD39L1, or rhCD39L3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENTPD5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L4/ENTPD5 Antibody (743512)

• CD39 antigen-like 4
• CD39L4
• CD39L4NTPDase 5
• CD39-like 4
• EC 3.6.1
• EC 3.6.1.42
• EC 3.6.1.6
• ectonucleoside triphosphate diphosphohydrolase 5
• ENTPD5
• ER-UDPase
• GDPase ENTPD5
• Guanosine-diphosphatase ENTPD5
• MGC163357
• MGC163359
• NTPDase 5
• NTPDase-5
• Nucleoside diphosphatase
• Pcph proto-oncogene protein
• PCPH
• proto-oncogene CPH
• UDPase ENTPD5
• Uridine-diphosphatase ENTPD5

Background

CD39L4, also called ENTPD5 (ectonucleoside triphosphate diphosphohydrolase 5), ER-UDPase and  PCPH, is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation‑dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa sequence identity with mouse CD39L4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD39L4/ENTPD5 Antibody (MAB5297)(1)

Publication using MAB5297

• JJ Hsiao, MM Smits, BH Ng, J Lee, ME Wright Discovery proteomics defines androgen-regulated glycoprotein networks in prostate cancer cells, as well as putative biomarkers of prostatic diseases Scientific Reports, 2021-11-15;11(1):22208. 2021-11-15 [PMID: 34782677] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ENTPD5
• Uniprot:
  • O75356(Human)