CD39L2/ENTPD6 Antibody (774308) [Unconjugated]

CD39L2/ENTPD6 was detected in immersion fixed CHO-S transfected (positive) and absent in CHO-S wild type (negative) cells using Rat Anti-Mouse CD39L2/ENTPD6 Monoclonal Antibody (Catalog # MAB6288) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD39L2/ENTPD6 was detected in immersion fixed paraffin-embedded sections of Mouse Testis using Rat Anti-Mouse CD39L2/ENTPD6 Monoclonal Antibody (Catalog # MAB6288) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in late spermatids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: IHC, ICC/IF
• Clone: 774308
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CD39L2/ENTPD6 Antibody (774308) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived mouse CD39L2/ENTPD6
  Lys33-Leu455
  Accession # 742115
• Specificity: Detects mouse CD39L2/ENTPD6 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L2/ENTPD6 Antibody (774308) [Unconjugated]

• CD39 antigen-like 2
• CD39L2
• CD39L2NTPDase-6
• CD39-like 2
• dJ738P15.3
• DKFZp781G2277
• DKFZp781K21102
• EC 3.6.1.6
• ectonucleoside triphosphate diphosphohydrolase 6 (putative function)
• ectonucleoside triphosphate diphosphohydrolase 6 (putative)
• ectonucleoside triphosphate diphosphohydrolase 6
• ENTPD6
• FLJ36711
• IL-6SAG
• IL6ST2
• interleukin 6 signal transducer-2
• NTPD3
• NTPDase 6

Background

CD39L2, also known as ectonucleoside triphosphate diphosphohydrolase-6 (ENTPD-6) and NTPDase-6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells (1). This Type II membrane protein also exists as a soluble, secreted protein. CD39L2 displays a preference for the nucleoside-5’-diphosphates GDP and IDP over CDP and UDP (2). Nucleoside-5’-triphosphates are also hydrolyzed, but at much lower rates. CD39L2 is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells (3). CD39L2 may play a functional role in the regulation and recruitment of platelet activation in the heart. The cytoplasmic and transmembrane domains of rmCD39L2 were replaced with a signal sequence, resulting in the secretion of the recombinant protein.

1. Chadwick, B.P. and A.M. Frischauf (1998) Genomics. 50:357.
2. Hicks-Berger, C.A. et al. (2000) J.Biol. Chem. 275:34041.
3. Yeung, G. et al. (2000) Biochemistry. 39:12916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • 742115()