CD39/ENTPD1 Antibody (498403) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: Flow
• Clone: 498403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

CD39/ENTPD1 Antibody (498403) [Alexa Fluor® 647] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD39/ENTPD1
  Thr38-Val478
  Accession # P49961
• Specificity: Detects human CD39/ENTPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD39, recombinant human CD39L2, 3, or 4 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENTPD1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39/ENTPD1 Antibody (498403) [Alexa Fluor® 647]

• ADPase
• ATPDase
• ATP-diphosphatase
• CD39 antigen
• CD39
• CD39EC 3.6.1.5
• DKFZp686D194
• DKFZp686I093
• EC 3.6.1
• ecto-apyrase
• Ecto-ATP diphosphohydrolase 1
• Ecto-ATPase 1
• Ecto-ATPDase 1
• ectonucleoside triphosphate diphosphohydrolase 1
• ENTPD1
• FLJ40921
• FLJ40959
• Lymphoid cell activation antigen
• NTPD1
• NTPDase 1
• NTPDase-1

Background

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

1. Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
2. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
3. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
4. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
5. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
6. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD39/ENTPD1 Antibody (FAB4397R)(1)

Publication using FAB4397R

• Brzoska, T;Kaminski, TW;Katoch, O;Menchikova, EV;Alagbe, AE;Tashbook, SE;Tofovic, SP;Jonassaint, JC;St Croix, CM;Watkins, SC;Howe, S;Field, JJ;Seyerle, AA;Pradhan-Sundd, T;Laurie, C;Pankratz, ND;Smith, NL;Goode, EL;Pankow, JS;Kooperberg, C;Kato, GJ;Zhang, Y;Novelli, EM;Gladwin, MT;Jackson, EK;Nouraie, SM;Sundd, P; CD39 polymorphism enables lung thrombosis in sickle cell disease Nature communications 2026-01-14 [PMID: 41530145] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ENTPD1
• Uniprot:
  • P49961()