| Reactivity | Hu, Pm, PmSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-Fr, IHC-P |
| Clone | SPM554 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 646 |
| Immunogen | Intact human monocytes |
| Localization | Cell surface |
| Marker | Follicular Dendritic Cell Marker |
| Specificity | Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![CD35 Antibody (SPM554) [Janelia Fluor® 646]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CD35 Antibody (SPM554) [Janelia Fluor® 646]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-34796AF350 | |
| Alexa Fluor 405 | NBP2-34796AF405 | |
| Alexa Fluor 488 | NBP2-34796AF488 | |
| Alexa Fluor 532 | NBP2-34796AF532 | |
| Alexa Fluor 594 | NBP2-34796AF594 | |
| Alexa Fluor 647 | NBP2-34796AF647 | |
| Alexa Fluor 700 | NBP2-34796AF700 | |
| Alexa Fluor 750 | NBP2-34796AF750 | |
| Allophycocyanin | NBP2-34796APC | |
| Biotin | NBP2-34796B | |
| DyLight 350 | NBP2-34796UV | |
| DyLight 405 | NBP2-34796V | |
| DyLight 488 | NBP2-34796G | |
| DyLight 550 | NBP2-34796R | |
| DyLight 594 | NBP2-34796DL594 | |
| DyLight 650 | NBP2-34796C | |
| DyLight 680 | NBP2-34796FR | |
| DyLight 755 | NBP2-34796IR | |
| FITC | NBP2-34796F | |
| HRP | NBP2-34796H | |
| Janelia Fluor 549 | NBP2-34796JF549 | |
| Janelia Fluor 646 | NBP2-34796JF646 | |
| PE | NBP2-34796PE | |
| PerCP | NBP2-34796PCP |
Diseases for CD35 Antibody (NBP2-34796JF646)Discover more about diseases related to CD35 Antibody (NBP2-34796JF646).
| Pathways for CD35 Antibody (NBP2-34796JF646)View related products by pathway.
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PTMs for CD35 Antibody (NBP2-34796JF646)Learn more about PTMs related to CD35 Antibody (NBP2-34796JF646).
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How to identify B cell subsets using flow cytometry By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a... Read full blog post. |
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| Gene Symbol | CR1 |
![Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on different lysates using anti-CD21 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: Raji](http://images.novusbio.com/images2/CD21 Antibody (SC0681)-Western Blot-NBP2-67605-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/CD21-Antibody-(SC0681)-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0002.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/CD21-Antibody-(SC0681)-Immunohistochemistry-Paraffin-NBP2-67605-img0005.jpg)
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining in human lung and kidney tissues using anti-CD55 antibody. Corresponding CD55 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CD55-DAF-Antibody-Immunohistochemistry-Paraffin-NBP1-85466-img0007.jpg)
![Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/images2/CD55-DAF-Antibody-Immunohistochemistry-Paraffin-NBP1-85466-img0004.jpg)
![Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining of human lung shows high expression.](http://images.novusbio.com/images2/CD55-DAF-Antibody-Immunohistochemistry-Paraffin-NBP1-85466-img0005.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Image from product review by verified customer.](http://images.novusbio.com/images2/CD11b-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89474-img0010.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/CD11b-Antibody-Dual-RNAscope-ISH-IHC-NB110-89474-img0025.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (http://www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD11b-Antibody-Immunohistochemistry-NB110-89474-img0024.jpg)
![Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).](http://images.novusbio.com/images2/CD59-Antibody-(MEM-43)-Immunohistochemistry-Paraffin-NB500-330-img0009.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/images2/CD59-Antibody-(MEM-43)-Flow-Cytometry-NB500-330-img0007.jpg)
![Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/images2/CD59-Antibody-(MEM-43)-Immunohistochemistry-Frozen-NB500-330-img0008.jpg)
![Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.](http://images.novusbio.com/images2/Complement-C4b-d-Antibody-(16D2)-Western-Blot-NB200-541-img0001.jpg)
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![Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).](http://images.novusbio.com/images2/CapG-Antibody-Western-Blot-NBP1-90214-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CapG Antibody [NBP1-90214] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/images2/CapG-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90214-img0008.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody (A) NBP1-90214 shows similar protein distribution across tissues to independent antibody NBP1-90215 (B).](http://images.novusbio.com/images2/CapG Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0021.jpg)
