CD35 Antibody (31R)

Product Discontinued

CD35 Antibody (31R) Summary

• Immunogen: Human CD35
• Specificity: The monoclonal antibody 31R binds a single epitope of CR1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CD35 Antibody (31R)

• C3BR
• CD35
• complement component (3b/4b) receptor 1 (Knops blood group)
• CR1
• KN

Background

CD35 (CR1) is a type 1 membrane glycoprotein participating in clearance of immune complexes, regulation of autologous complement activation on cell surfaces, and complement-dependent cellular cytotoxicity mediated by leukocytes. CD35 inhibits complement activation by two distinct mechanisms. CD35 is a cofactor for factor I cleavage of C3b, iC3b and C4b. Furthermore CD35 mediates decay-dissociation of C3 and C5 convertases. CD35 is found on erythrocytes, neutrophils, monocytes and dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CR1
• Entrez:
  • 1378(Human)