CD302/CLEC13A Antibody (771910) [Unconjugated]

Human PBMC were stained with (A) Mouse Anti-Human CD302/CLEC13A Monoclonal Antibody (Catalog # MAB6367) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and PE-conjugated Mouse anti-Human CD14 Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 771910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD302/CLEC13A Antibody (771910) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD302/CLEC13A
  Asp23-Asn167
  Accession # Q81X05
• Specificity: Detects human CD302/CLEC13A in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse CD302 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD302
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD302/CLEC13A Antibody (771910) [Unconjugated]

• BIMLEC
• CD302 antigenC-type lectin domain family 13, member A
• CD302 molecule
• CD302
• CLEC13A
• CLEC13AMGC22301
• C-type lectin domain family 13 member A
• DCL1
• DCL-1
• DCL1C-type lectin BIMLEC
• KIAA0022FLJ43091
• Type I transmembrane C-type lectin receptor DCL-1

Background

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein.  It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205.  CD302 contributes aa 23-232 to the fusion protein.  There is one potential alternative start site at Met63.  Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD302
• Uniprot:
  • Q81X05()