CD300a/LMIR1 Antibody (MEM-260) - BSA Free

Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) [NB110-58726] - staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.

Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) [NB110-58726] - Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: MEM-260
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD300a/LMIR1 Antibody (MEM-260) - BSA Free Summary

• Immunogen: HPB acute lymphoid leukemia cell line (NM_007261.2).
• Specificity: This antibody (clone MEM-260) reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD300A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Western Blot 1:1000
• Application Notes: Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. This antibody is CyTOF ready.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CD300a/LMIR1 Antibody (MEM-260) - BSA Free

• CD300a antigenCMRF35H9
• CD300a molecule
• CD300a
• CLM8
• CLM-8
• CMRF35H leukocyte immunoglobulin-like receptor
• CMRF-35H
• CMRF35-H
• CMRF35-H9
• CMRF-35-H9CD300 antigen-like family member A
• CMRF35HIRp60
• CMRF35-like molecule 8
• IGSF12
• IGSF12NK inhibitory receptor
• Immunoglobulin superfamily member 12
• Inhibitory receptor protein 60
• IRC1
• IRC1/IRC2
• IRC2
• IRp60
• leukocyte membrane antigen
• LMIR1
• MAIR-I
• Mcipr1
• NKRL
• Pigr4

Background

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD300a/LMIR1 Antibody (NB110-58726)(1)

Publication using NB110-58726

• Larsen LF, Juel-Berg N, Hansen A et al. No difference in human mast cells derived from peanut allergic versus non-allergic subjects Immun Inflamm Dis. 2018-12-06 [PMID: 29992767] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.

Bioinformatics

• Gene Symbol: CD300A
• Entrez:
  • 11314(Human)
• Uniprot:
  • Q9UGN4()