CD30/TNFRSF8 Antibody (CD30/412)

Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (CD30/412) [NBP2-29425] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma (20X) stained with CD30 Ab (CD30/412).

Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (CD30/412) [NBP2-29425] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma(20X) stained with CD30 Ab (CD30/412).

• Reactivity: Hu
• Applications: IHC
• Clone: CD30/412
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD30/TNFRSF8 Antibody (CD30/412) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33196)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human CD30/TNFRSF8 recombinant protein (Uniprot: P28908)
• Localization: Cell surface
• Marker: Hodgkin & Reed-Sternberg Cell Marker
• Specificity: Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkins disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This monoclonal antibody distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD30/TNFRSF8 Antibody (CD30/412)

• CD30 antigen
• CD30
• CD30KI-1
• CD30L receptor
• cytokine receptor CD30
• D1S166EKi-1
• Ki-1 antigen
• Lymphocyte activation antigen CD30
• TNFRSF8
• tumor necrosis factor receptor superfamily member 8
• tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a transmembrane cytokine receptor belonging to the tumour necrosis factor (TNF) receptor superfamily. It is expressed in carcinoma but not in seminoma (testicular cancer) making the CD30 C30/412 antibody a useful tool in distinguishing between these germ cell tumors. CD30 is also expressed on classical Hodgkin's lymphoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD30/TNFRSF8 Antibody (NBP2-29425)(1)

Publication using NBP2-29425

• Hartmann S, Scharf S, Steiner Y et al. Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas Cancers (Basel) 2021-10-17 [PMID: 34680356]
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.

Bioinformatics

• Gene Symbol: TNFRSF8
• Uniprot:
  • P28908()
  • P28908()
  • P28908()