Recombinant Human CD23/Fc epsilon RII Protein

SDS-Page: Recombinant Human CD23/Fc epsilon RII Protein [NBP2-35242]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CD23/Fc epsilon RII Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 172 amino acids corresponding to CD23/Fc epsilon RII Source: E. coli

  Uniprot ID: P06734

  Amino Acid Sequence: MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS

• Details of Functionality: CD23 Protein is fully biologically active when compared to standard. The ED50 determined by its ability to induce TNF-alpha production by human PBMCs.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FCER2
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CD23/Fc epsilon RII as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CD23/Fc epsilon RII Protein

• BLAST-2
• CD23
• CD23A
• CD23CD23 antigen
• CLEC4J
• CLEC4JC-type lectin domain family 4 member J
• C-type lectin domain family 4, member J
• Fc epsilon RII
• Fc fragment of IgE, low affinity II, receptor for (CD23)
• FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A)
• Fc-epsilon-RII
• FCER2
• Fcer2a
• FceRII
• IGEBF
• Immunoglobulin E-binding factor
• low affinity immunoglobulin epsilon Fc receptor
• Ly-42
• Lymphocyte IgE receptor

Background

CD23 is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FCER2
• Uniprot:
  • P06734()
  • P06734()
  • P06734()