Human CD23/Fc epsilon RII ELISA Kit (Colorimetric)

Human CD23/Fc epsilon RII ELISA Kit [NBP1-91173] - Data for Human sCD23 Platinum ELISA

Product Discontinued

Human CD23/Fc epsilon RII ELISA Kit (Colorimetric) Summary

• Description: The human sCD23 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD23
• Specificity: The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD23/Fc epsilon RII positive serum. There was no cross reactivity detected.
• Standard Curve Range: 12.5 - 400 U/mL
• Sensitivity: 6.8 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.30%
• Intra-Assay: CV% < 4.00%
• Spike Recovery: The recovery ranged from 81% to 120% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FCER2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD23/Fc epsilon RII ELISA Kit (Colorimetric)

• BLAST-2
• CD23
• CD23A
• CD23CD23 antigen
• CLEC4J
• CLEC4JC-type lectin domain family 4 member J
• C-type lectin domain family 4, member J
• Fc epsilon RII
• Fc fragment of IgE, low affinity II, receptor for (CD23)
• FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A)
• Fc-epsilon-RII
• FCER2
• Fcer2a
• FceRII
• IGEBF
• Immunoglobulin E-binding factor
• low affinity immunoglobulin epsilon Fc receptor
• Ly-42
• Lymphocyte IgE receptor

Background

CD23 is described as a 45 kDa protein found on the surface of IgM bearing B cells, eosinophils, macrophages and some T and NK cells. It is also found on EBV-transformed B cells. Additionally, a released form has been described. When first released the CD23 molecule is 35 kDa; however, this form is quickly cleaved to obtain the more stable, soluble form which is 25 kDa in size.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD23/Fc epsilon RII ELISA Kit (NBP1-91173). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FCER2