Human CD23/Fc epsilon RII ELISA Kit (Colorimetric) Summary
| Description |
The human sCD23 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD23 |
| Specificity |
The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD23/Fc epsilon RII positive serum. There was no cross reactivity detected. |
| Standard Curve Range |
12.5 - 400 U/mL |
| Sensitivity |
6.8 U/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 6.30% |
| Intra-Assay |
CV% < 4.00% |
| Spike Recovery |
The recovery ranged from 81% to 120% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details. |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
FCER2 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD23
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human sCD23 monoclonal antibody
- Control high, lyophilized
- Control low, lyophilized
- Human sCD23 Standard lyophilized, 400 U/ml upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Human CD23/Fc epsilon RII ELISA Kit (Colorimetric)
Background
CD23 is described as a 45 kDa protein found on the surface of IgM bearing B cells, eosinophils, macrophages and some T and NK cells. It is also found on EBV-transformed B cells. Additionally, a released form has been described. When first released the CD23 molecule is 35 kDa; however, this form is quickly cleaved to obtain the more stable, soluble form which is 25 kDa in size.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CD23/Fc epsilon RII ELISA Kit (NBP1-91173). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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