CD23/Fc epsilon RII Antibody (691632) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
691632
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

CD23/Fc epsilon RII Antibody (691632) [Alexa Fluor® 350] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD23/Fc epsilon RII
Glu50-Pro331
Accession # P20693
Specificity
Detects mouse CD23/Fc epsilon RII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc epsilon RIA or rhFc epsilon RIG is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Fcer2a
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD23/Fc epsilon RII Antibody (691632) [Alexa Fluor® 350]

  • BLAST-2
  • CD23
  • CD23A
  • CD23CD23 antigen
  • CLEC4J
  • CLEC4JC-type lectin domain family 4 member J
  • C-type lectin domain family 4, member J
  • Fc epsilon RII
  • Fc fragment of IgE, low affinity II, receptor for (CD23)
  • FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A)
  • fc-epsilon-RII
  • FCER2
  • Fcer2a
  • FceRII
  • IGEBF
  • Immunoglobulin E-binding factor
  • low affinity immunoglobulin epsilon Fc receptor
  • Ly-42
  • Lymphocyte IgE receptor

Background

The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2).  Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

  1. Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
  2. Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
  3. Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
  4. Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
  5. Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
  6. Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
  7. Sukumar, S. et al. (2006) J. Immunol. 176:4811.
  8. Karagiannis, S.N. et al. (2001) Immunology 103:319.
  9. Aubry, J-P. et al. (1992) Nature 358:505.
  10. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
  11. Montagnac, G. et al. (2005) Traffic 6:230.
  12. Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
  13. Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
  14. Entrez Accession # EDL21949, EDL21951.
  15. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6900U
Alexa Fluor 405 FAB6900V
Alexa Fluor 488 FAB6900G
Alexa Fluor 647 FAB6900R
Alexa Fluor 700 FAB6900N
Alexa Fluor 750 FAB6900S

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Bioinformatics

Gene Symbol Fcer2a
Uniprot
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