CD177 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

CD177 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD177
  Leu22-Gly407
  Accession # AAH29167
• Specificity: Detects human CD177 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD177 Antibody [Alexa Fluor® 750]

• CD177 antigenNB1 GP
• CD177 molecule
• CD177
• HNA-2a
• HNA2Acell surface receptor
• Human neutrophil alloantigen 2a
• NB1 GP
• NB1PRV-1
• polycythemia rubra vera 1
• Polycythemia rubra vera protein 1
• PRV1
• PRV1NB1 glycoprotein

Background

CD177 is a polymorphic gene in the leukocyte antigen (Ly6) uPAR superfamily. It encodes at least two alleles of the neutrophilic membrane protein PRV1 (Polycythemic Rubra Vera protein 1) and NB1 (also named HNA-2a) that are glycosyl-phosphotidylinositol (GP1) anchored proteins of 58‑64 kDa. CD177 has two highly homologous cysteine-rich uPAR/Ly6 domains. The mRNA levels of CD177 is elevated under conditions where neutrophil counts are increased and is a useful marker for polycythemic vera. Mature human and mouse CD177 share 43% amino acid sequence identity. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (1-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.