CD177 Antibody (MEM-166)

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Flow Cytometry: CD177 Antibody (MEM-166) [NB100-77971] - Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clone
MEM-166
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CD177 Antibody (MEM-166) Summary

Immunogen
Human granulocytes
Specificity
CD177 (MEM-166)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD177
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunoprecipitation
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
CD177 Lysate (NBL1-08911)

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Rhesus.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD177 Antibody (MEM-166)

  • CD177 antigenNB1 GP
  • CD177 molecule
  • CD177
  • HNA-2a
  • HNA2Acell surface receptor
  • Human neutrophil alloantigen 2a
  • NB1 GP
  • NB1PRV-1
  • polycythemia rubra vera 1
  • Polycythemia rubra vera protein 1
  • PRV1
  • PRV1NB1 glycoprotein

Background

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils. The MEM-166 antibody is useful for flow cytometry, immunoprecipitation, and Western blotting under non-reducing conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD177 Antibody (NB100-77971) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD177
Entrez