CD160 Antibody

Mouse splenocytes (CD8-gated) stimulated with LPS for 3 hours were stained with Sheep Anti-Mouse CD160 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3899) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Rat Anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

CD160 was detected in immersion fixed mouse CD8+ splenocytes using Sheep Anti-Mouse CD160 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3899) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD160 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD160
  Gly28-Ser160
  Accession # AAH21596
• Specificity: Detects mouse CD160 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD160 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CD160
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD160 Antibody

• BY55
• BY55FLJ46513
• CD160 antigenimmunoglobulin superfamily member
• CD160 molecule
• CD160
• Natural killer cell receptor BY55
• NK1
• NK28

Background

-CD160 (also Natural killer cell receptor BY55) is a 16 kDa (predicted) member of the Ig superfamily (1 - 4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ T EM and T CM T cells, CD8 alpha + IELs, NKT cells, CD8-gamma δ TCR T cells, and vascular endothelial cells (1, 5-7). Mouse CD160 has been identified as a 20-21 kDa GPI-linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185 amino acids (aa) in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V-type Ig-like domain (aa 28-125), and a 25 aa prosegment that is cleaved to generate a GPI-linkage at Ser160. Mouse GPI-linked CD160 is known to be cleaved by phospholipase C, and this generates a 40 kDa (presumably dimeric) band in SDS-PAGE (5). One alternative splice form for mouse CD160 is reported that appears to show a deletion of aa 137-180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively. In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non-classical MHC Class I molecules (5, 8). MHC-I proteins recognized by CD160 include Dd, Kb, Qa-1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN-gamma secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).

1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
5. Maeda, M. et al. (2005) J. Immunol. 175:4426.
6. Fons, P. et al. (2006) Blood 108:2608.
7. Tsujimura, K. et al. (2006) Immunol. Lett. 48:106.
8. Cheung, T.C. et al. (2009) Proc. Natl. Acad. Sci USA 106:6244.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD160 Antibody (AF3899)(2)

Publications using AF3899

• Sophie Flommersfeld, Jan P. Böttcher, Jonatan Ersching, Michael Flossdorf, Philippa Meiser, Ludwig O. Pachmayr et al. Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection Immunity 2021-10-12 [PMID: 34437840] (Flow Cytometry, Mouse)
• Jiansong Wu, Qiang Niu, Jie Yuan, Xiaodan Xu, Liuxia Cao lncRNA‑CD160 decreases the immunity of CD8+ T cells through epigenetic mechanisms in hepatitis B virus infection Oncology Letters 2020-04-15 [PMID: 32565950] (Flow Cytometry, Immunohistochemistry, Human, Mouse)

Bioinformatics

• Gene Symbol: CD160
• Uniprot:
  • AAH21596()