CD155/PVR Antibody (1072806) [Unconjugated]

CD155/PVR was detected in fixed HT‑29 human colon adenocarcinoma cell line (Positive) and absent in Daudi human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB11503) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

U937 cells were stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB11503, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 1072806
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD155/PVR Antibody (1072806) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD155/PVR
  Gly27-Asn343
  Accession # P15151
• Specificity: Detects human CD155/PVR in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD155/PVR Antibody (1072806) [Unconjugated]

• CD155 antigen
• CD155
• HVED
• NECL5
• Necl-5
• nectin-like 5
• Nectin-like protein 5
• poliovirus receptor
• PVR
• PVS
• PVSFLJ25946
• Tage4

Background

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

1. Takai, Y. et al. (2003) Cancer Sci. 94:655.
2. Mendelsohn, C.L. et al. (1989) Cell 56:855.
3. Koike, H. et al. (1990) EMBO J. 9:3217.
4. Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
5. Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
6. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
8. Lange, R. et al. (2001) Virology 285:218.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P15151(Human)