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CCT6A Antibody

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Western Blot: CCT6A Antibody [NBP2-92778] - Western blot analysis of extracts of Mouse brain, using CCT6A antibody (NBP2-92778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: CCT6A Antibody [NBP2-92778] - Western blot analysis of extracts of Mouse brain, using CCT6A antibody (NBP2-92778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CCT6A Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-250 of human CCT6A (NP_001753.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCT6A
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CCT6A Antibody

  • acute morphine dependence related protein 2
  • Acute morphine dependence-related protein 2
  • amino acid transport defect-complementing
  • CCT6
  • Cctz
  • CCT-zeta
  • CCT-zeta-1
  • chaperonin containing T-complex subunit 6
  • chaperonin containing TCP1, subunit 6A (zeta 1)
  • histidine transport regulator 3
  • HTR3MGC126215
  • MGC126214
  • MoDP-2
  • T-complex protein 1 subunit zeta
  • T-complex protein 1, zeta subunit
  • TCP-1-zeta
  • TCP20
  • TCPZ
  • TTCP20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1400
Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
HSP60 Antibody -
BSA Free
NBP1-77397
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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(3 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
SPINK1 Antibody
(4D4)
H00006690-M01
Western Blot: SPINK1 Antibody (4D4) [H00006690-M01] - Detection against Immunogen (31.9 kDa). Antibody reactive against recombinant protein.
Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Representative images for immunohistochemical staining of SPINK1in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20mg/kg body weight) or vehicle control (n=5 each). Intact group represents non-castrated control mice (n=5). Scale bar represents 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY licence.
Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Representative images showing H&E staining (x200 magnification) and immunostaining (x200 magnification) for AR, synaptophysin, and SPINK1 in tumor specimens obtained from NEPC patients', namely WCM12, WCM155 (an organoid), and WCM677. Scale bar represents 100um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY licence.
(1 Review)
(27 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
TCP1-beta Antibody
NBP1-88146
Immunocytochemistry/Immunofluorescence: TCP1-beta Antibody [NBP1-88146] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human testis shows high expression.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
5-HT3A Antibody
NB100-56351
Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (http://journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY licence.
Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB
CCT3 Antibody
NBP1-86562
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-86562] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP1-86562] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
MARVELD2 Antibody
NB100-74633
Western Blot: MARVELD2 Antibody [NB100-74633] - Detection of Human MARVELD2/Tricellulinon HeLa whole cell lysate using NB100-74633. MARVELD2/Tricellulin was also immunoprecipitated by rabbit anti-MARVELD2/Tricellulin antibody NB100-74632.
(2 Publications)
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Applications: ICC/IF, IHC, IHC-Fr, IP, WB
UCK2 Antibody
(1G12)
H00007371-M03
Sandwich ELISA: UCK2 Antibody (1G12) [H00007371-M03] - Detection limit for recombinant GST tagged UCK2 is 1 ng/ml as a capture antibody.
Western Blot: UCK2 Antibody (1G12) [H00007371-M03] - Analysis of UCK2 transfected lysate using anti-UCK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UCK2 rabbit polyclonal antibody.
Species: Hu
Applications: ELISA, IP, S-ELISA, WB
CCT5 Antibody
NBP1-83042
Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-83042] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human testis shows high expression.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TCP1-delta Antibody
NBP1-88145
Immunocytochemistry/Immunofluorescence: TCP1-delta Antibody [NBP1-88145] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: TCP1-delta Antibody [NBP1-88145] - Staining in human testis and pancreas tissues using anti-CCT4 antibody. Corresponding CCT4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TCP1-delta Antibody [NBP1-88145] - Staining of human pancreas shows low expression as expected.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
5-HT3B Antibody
(2H9H5) - BSA Free
NBP2-61787
ELISA: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
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Applications: ELISA, Flow, ICC/IF, WB
PCYT1B Antibody
H00009468-D01P
Western Blot: PCYT1B Antibody [H00009468-D01P] - Analysis of PCYT1B expression in MCF-7.
Western Blot: PCYT1B Antibody [H00009468-D01P] - Analysis of PCYT1B expression in transfected 293T cell line by PCYT1B polyclonal antibody.Lane 1: PCYT1B transfected lysate(41.90 KDa).Lane 2: Non-transfected lysate.
Western Blot: PCYT1B Antibody [H00009468-D01P] - Analysis of PCYT1B expression in human pancreas.
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Applications: WB
CCT6B Antibody
NBP2-92177
Immunocytochemistry/Immunofluorescence: CCT6B Antibody [NBP2-92177] - Analysis of HeLa cells using CCT6B at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: CCT6B Antibody [NBP2-92177] - Analysis of extracts of various cell lines, using CCT6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunocytochemistry/Immunofluorescence: CCT6B Antibody [NBP2-92177] - Analysis of L929 cells using CCT6B at dilution of 1:100. Blue: DAPI for nuclear staining.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
PCYT1A Antibody
NBP1-84366
Immunocytochemistry/Immunofluorescence: PCYT1A Antibody [NBP1-84366] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: PCYT1A Antibody [NBP1-84366] - Analysis in human cell line EFO-21.
Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP1-84366] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
5-HT3E Antibody
NBP2-82540
Western Blot: 5-HT3E Antibody [NBP2-82540] - WB Suggested Anti-HTR3E Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart
Immunohistochemistry: 5-HT3E Antibody [NBP2-82540] - Immunofluorescence -- Dilution: 1.3ug/mL
Species: Hu, Mu
Applications: ICC/IF, IHC, WB
TCP1-eta Antibody
(1D6)
H00010574-M01
Western Blot: TCP1-eta Antibody (1D6) [H00010574-M01] - Western Blot analysis of CCT7 expression in K-562 ( Cat # L009V1 ).
ELISA: TCP1-eta Antibody (1D6) [H00010574-M01] - Detection limit for recombinant GST tagged CCT7 is approximately 0.3ng/ml as a capture antibody.
Western Blot: TCP1-eta Antibody (1D6) [H00010574-M01] - CCT7 monoclonal antibody (M01), clone 1D6. Analysis of CCT7 expression in PC-12.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, WB

Publications for CCT6A Antibody (NBP2-92778) (0)

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Bioinformatics

Gene Symbol CCT6A