CCT5 Antibody (639) - Azide and BSA Free

Western Blot: CCT5 Antibody (639) [NBP2-43680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCT5 Antibody (639) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CCT5 Antibody (639) [NBP2-43680] - CCT5 Antibody (639) detects CCT5 protein at centrosome and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CCT5 stained by CCT5 Antibody (639) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Western Blot: CCT5 Antibody (639) [NBP2-43680] - Analysis of various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with TCP1 epsilon antibody diluted by 1:1000.

Western Blot: CCT5 Antibody (639) [NBP2-43680] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [639].

Immunoprecipitation: CCT5 Antibody (639) [NBP2-43680] - Analysis of TCP1 epsilon protein from 293T whole cell extracts using 5 ug of TCP1 epsilon antibody [639]. Western blot analysis was performed using TCP1 epsilon antibody [639]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Ha, Rt, Po, Bv, Pm, RM
• Applications: WB, ICC/IF, IP
• Clone: 639
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CCT5 Antibody (639) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CCT5 Antibody (639) - Azide and BSA Free (NBP2-43680) is a monoclonal antibody validated for use in WB, ICC/IF and IP. Anti-CCT5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CCT5. The exact sequence is proprietary.
• Predicted Species: Rat (94%), Porcine (96%), Chimpanzee (100%), Bovine (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCT5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31964905). Zebrafish (83%), Xenopus laevis (86%), Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for CCT5 Antibody (639) - Azide and BSA Free

• chaperonin containing TCP1, subunit 5 (epsilon)
• epsilon subunit

Background

CCT5 encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCT5 Antibody (NBP2-43680)(1)

Publication using NBP2-43680

• Showalter AE, Martini AC, Nierenberg D et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis Sci Rep 2020-01-21 [PMID: 31964905] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: CCT5