CCL8/MCP-2 Antibody [Unconjugated]

Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP‑2 Polyclonal Antibody (Catalog # AB-281-NA). The ND50 is typically 60‑120 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL8/MCP-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL8/MCP-2
  Gln24-Pro99
  Accession # P80075
• Specificity: Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) MCP-1 and rhMCP-3 is observed. Neutralizes the biological activity of recombinant human MCP-2, but will not neutralize the biological activity of MCP-1 or MCP-3.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL8
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 60-120 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL8/MCP-2 Antibody [Unconjugated]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL8/MCP-2 Antibody (AB-281-NA)(1)

Publication using AB-281-NA

• Gavin M. Mason, Emma Poole, J. G. Patrick Sissons, Mark R. Wills, John H. Sinclair Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function Proceedings of the National Academy of Sciences 2012-09-04 [PMID: 22826250]

Bioinformatics

• Gene Symbol: CCL8
• Uniprot:
  • P80075()