Mouse CCL12/MCP-5 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1801
    • Availability
      Product Discontinued

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Mouse CCL12/MCP-5 ELISA Kit (Colorimetric) Summary

Sensitivity
0.5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
Ccl12

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Ccl12 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Ccl12.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasm a) diluent.
  2. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent.
  3. Detection Antibody MCP-5 (Item F): 2 vial of biotinylated anti-mouse MCP-5 (each vial is enough to assay half microplate)
  4. HRP-Streptavidin Concentrate (Item G): 8 µl 8,000x concentrated HRP-conjugated streptavidin.
  5. MCP-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse MCP-5.
  6. Standards (Item C): 2 vials of recombinant mouse MCP-5.
  7. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse CCL12/MCP-5 ELISA Kit (Colorimetric)

  • C-C motif chemokine 12
  • CCL12
  • chemokine (C-C motif) ligand 12
  • MCP5
  • MCP-5
  • Scya12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CCL12/MCP-5 ELISA Kit (KA1801). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol Ccl12