CCL12/MCP-5 Antibody (147801) [Unconjugated]

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428‑P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB4281). The ND50 is typically 0.5-2.5 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 147801
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL12/MCP-5 Antibody (147801) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL12/MCP‑5
  Gly23-Gly104
  Accession # Q62401
• Specificity: Detects mouse CCL12/MCP‑5 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-2.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL12/MCP-5 Antibody (147801) [Unconjugated]

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104 amino acid (aa) precursor protein with a 22 aa signal sequence that is cleaved to generate a 82 aa mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ccl12
• Uniprot:
  • Q62401()