CCK-AR Antibody

Product Discontinued

CCK-AR Antibody Summary

• Immunogen: Peptide with sequence C-KFDASQKKSAKEKR corresponding to internal region according to Uniprot Mouse NP_033957.1.
• Epitope: KFDASQKKSAKEKR
• Predicted Species: Mouse (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCKAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in purified membranes of rat insulinoma cell line RIN14B (PMID: 20011057). The observed MW also corresponds to earlier findings in scientific literature with different antibodies (PMID: 14566022).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCK-AR Antibody

• CCK1R
• CCK1-R
• CCK-A R
• CCK-A receptor
• CCK-A
• CCKAR
• CCK-AR
• CCKRA
• cholecystokinin A receptor
• cholecystokinin receptor type A
• cholecystokinin type-A receptor
• Cholecystokinin-1 receptor

Background

The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCK-AR Antibody (NB100-60552)(2)

Publications using NB100-60552

• Bi S, Scott KA, Kopin AS, Moran TH. Differential roles for cholecystokinin a receptors in energy balance in rats and mice. Endocrinology 2004-08-01 [PMID: 15123537]
• Morisset J, Julien S, Laine J. Localization of cholecystokinin receptor subtypes in the endocine pancreas. J Histochem Cytochem 2003-11-01 [PMID: 14566022]

Bioinformatics

• Gene Symbol: CCKAR
• Entrez:
  • 12425(Mouse)
  • 24889(Rat)
• Uniprot:
  • NP_033957.1(Mouse)