FAQs for CBX4 Antibody (NBP1-83225). (Showing 1 - 2 of 2 FAQs).
I learn from your company web that you are providing atf6, xbp1, cbx4 antibodies against human for immunofluorescence. And from the provided pictures, it seems very good. But I am wondering if anyone use them for immunofluorescence in human cells in your memory or in published papers? And how about them? By the way, you are providing free sample size for the atf6, xbp1 antibodies, aren't you? Could you send me some for tests? One more question, do they work on endogenous proteins but not only on overexpressed proteins?
These products have been validated for use in IF on human: catalog numbers NBP1-40256, NB100-80861, and NBP1-83225. Yes these antibodies should work on the native protein/endogenous protein.
We tested WB with NBP1-83225. The MW of this item was around 80kDa in the website. But we saw the result of 60kDa. When she searched paper and the other brand's datasheet, MW of CBX4 was 60kDa. We want to know the reason the difference from yours. Could you explain about it?
The predicted molecular weight of Human CBX4 protein is 61.6kDa. There are a number of reasons why predicted protein size does not always look that way on a Western blot. One has to consider post-translational modifications, splice variants, tissue specific processing, multimer formation and amino acid charge. To confirm specificity, a control such as recombinant protein or overexpression lysate, a downregulated knockdown/knockout lysate, a peptide competition of the primary antibody, or a treatment known to effect the expression of the protein should be used.
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