Recombinant Human CBR1 Protein

SDS-Page: Recombinant Human CBR1 Protein [NBC1-26370] - CBR1, 30 kDa (277aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human CBR1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-277 of Human CBR1
  
  

  Source: E.coli

  Amino Acid Sequence: MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET PVYLALLPPD AEGPHGQFVS EKRVEQW

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CBR1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.5), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human CBR1 Protein

• 15-hydroxyprostaglandin dehydrogenase [NADP+]
• carbonyl reductase (NADPH) 1
• carbonyl reductase (NADPH) 1, EC 1.1.1.18410EC 1.1.1.197,15-hydroxyprostaglandin dehydrogenase
• carbonyl reductase [NADPH] 1
• carbonyl reductase 1
• CBR
• CRN
• EC 1.1.1.184
• EC 1.1.1.189
• hCBR1
• NADPH-dependent carbonyl reductase 1
• Prostaglandin 9-ketoreductase
• Prostaglandin-E(2) 9-reductase
• SDR21C1
• short chain dehydrogenase/reductase family 21C, member 1

Background

CBR1(Carbonyl reductase 1) is one of several monomeric NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. CBR1 is widely distributed in human tissues. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer doxorubicin. CBR1 can also convert prostaglandin E2 to prostaglandin F2-alpha. Recombinant human CBR1 protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CBR1