CBP20 Overexpression Lysate (Native)


Western Blot: CBP20 Overexpression Lysate (Adult Normal) [NBL1-13507] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CBP20.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

CBP20 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201519 and based on accession number NM_007362. The protein contains a C-terminal DDK tag.
Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for CBP20

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CBP20 Overexpression Lysate (Native)

  • 20 kDa nuclear cap-binding protein
  • Cbc2
  • CBP20CBC2
  • NCBP 20 kDa subunit
  • NCBP interacting protein 1
  • NCBP-interacting protein 1
  • NIP1Cell proliferation-inducing gene 55 protein
  • nuclear cap binding protein subunit 2, 20kD
  • nuclear cap binding protein subunit 2, 20kDa
  • nuclear cap-binding protein subunit 2


The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CBP20 Lysate (NBL1-13507) (0)

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol NCBP2

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