CBP20 Overexpression Lysate (Native)

Images

 
Western Blot: CBP20 Overexpression Lysate (Adult Normal) [NBL1-13507] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CBP20.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CBP20 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201519 and based on accession number NM_007362. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1, mRNA.
Gene
NCBP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CBP20

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CBP20 Overexpression Lysate (Native)

  • 20 kDa nuclear cap-binding protein
  • Cbc2
  • CBP20CBC2
  • NCBP 20 kDa subunit
  • NCBP interacting protein 1
  • NCBP-interacting protein 1
  • NIP1Cell proliferation-inducing gene 55 protein
  • nuclear cap binding protein subunit 2, 20kD
  • nuclear cap binding protein subunit 2, 20kDa
  • nuclear cap-binding protein subunit 2

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CBP20 Lysate (NBL1-13507) (0)

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Bioinformatics

Gene Symbol NCBP2