Cav1.2 Antibody

Product Discontinued

Cav1.2 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of rat CACNA1C (within residues 1150-1200). [Swiss-Prot# P22002]
• Localization: Membrane; Multi-pass membrane protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNA1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Cav1.2 antibody is useful for Blocking/Neutralizing, Western blot and Immunoprecipitation. In WB a band can be seen at ~250 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, rabbit and other vertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cav1.2 Antibody

• alpha-1 subunit
• CACH2
• CACN2
• CACNA1C
• CACNL1A1
• calcium channel, voltage-dependent, L type, alpha 1C subunit
• cardic dihydropyridine-sensitive, alpha-1 subunit
• Cav1.2
• CCHL1A1
• L type, alpha 1 polypeptide, isoform 1, cardic muscle
• L type, alpha-1 polypeptide, isoform 1, cardiac muscle
• splice variant 10*

Background

CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by CACNA1C this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cav1.2 Antibody (NBP1-42820)(1)

Publication using NBP1-42820

• Eden M, Meder B, Volkers M et al. Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression. Nat Commun. 2016-04-28 [PMID: 27122098] (WB, IP, Mouse, Human, Rat)

FAQs for Cav1.2 Antibody (NBP1-42820). (Showing 1 - 1 of 1 FAQ).

1. For the anti-Cav1.2 antibody NBP1-42820, would you be able to provide additional details regarding the testing done in WB applications? 
   • This antibody was tested in western blot with NG108 cell lysate. The protein is also expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle tissue. Lysates from these tissues should provide a good positive control for testing this product.

Bioinformatics

• Gene Symbol: CACNA1C
• Entrez:
  • 775(Human)
  • 12288(Mouse)
  • 24239(Rat)
• Uniprot:
  • Q13936(Human)
  • Q01815(Mouse)
  • P22002(Rat)