Cav1.2 Antibody (S57-46)

Images

 
Western Blot: Cav1.2 Antibody (S57-46) [NBP1-22439] - analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46. ...read more
Immunohistochemistry-Paraffin: Cav1.2 Antibody (S57-46) [NBP1-22439] - Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody at 1:10000 for 12 ...read more
Western Blot: Cav1.2 Antibody (S57-46) [NBP1-22439] - analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46. Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium ...read more
Immunohistochemistry: Cav1.2 Antibody (S57-46) [NBP1-22439] - Human hippocampus tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
S57-46
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Cav1.2 Antibody (S57-46) Summary

Immunogen
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
Localization
Membrane, Cell Membrane
Specificity
Detects approx 240kDa (varies with cell background due to glycosylation).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CACNA1C
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Cav1.2 Antibody was sufficient for detection of Cav1.2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publication using
NBP1-22439 in the following applications:

  • WB
    1 publication

Reactivity Notes

Based on homology, It's predicted to detect Hamster and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Cav1.2 Antibody (S57-46)

  • alpha-1 subunit
  • calcium channel, voltage-dependent, L type, alpha 1C subunit
  • cardic dihydropyridine-sensitive, alpha-1 subunit
  • L type, alpha 1 polypeptide, isoform 1, cardic muscle
  • L type, alpha-1 polypeptide, isoform 1, cardiac muscle
  • splice variant 10*

Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on an ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav1.2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart (3). It may be associated with a variant of Long QT syndrome called Timothy's syndrome (4, 5) and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cav1.2 Antibody (NBP1-22439)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-22439AF405
Alexa Fluor 488 Labeled NBP1-22439AF488
Alexa Fluor 647 Labeled NBP1-22439AF647
Alexa Fluor 700 Labeled NBP1-22439AF700
DyLight 350 Labeled NBP1-22439UV
DyLight 405 Labeled NBP1-22439V
DyLight 488 Labeled NBP1-22439G
DyLight 680 Labeled NBP1-22439FR
DyLight 755 Labeled NBP1-22439IR

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Bioinformatics

Gene Symbol CACNA1C
Entrez
Uniprot